【摘要】　目的　研究牛颈静脉带瓣管道经不同方法交联后免疫原性变化。方法　牛颈静脉随机分为4组：新鲜牛颈静脉对照组，多聚环氧化合物（PC）交联组、戊二醛（GA）交联组、光氧化（PH）交联组。牛颈静脉分别匀浆提取抗原，再与完全弗氏佐剂混合制成复合乳剂抗原后免疫新西兰大白兔，双向免疫扩散法测定兔血清抗体滴度。取新鲜牛颈静脉免疫抗血清采用免疫印迹法（Westernblot）体外检测交联后的牛颈静脉带瓣管道免疫原性。结果　新鲜牛颈静脉呈强抗原性，经PC、GA、PH处理的牛颈静脉抗原性显著性降低。结论 经PC、GA、PH适当交联处理的牛颈静脉免疫原性均显著性降低，各组间免疫原性无明显差异。 关键词　牛颈静脉 免疫原性 免疫印迹法 Research on immunogenicity of different cross-linking methods tanned Bovine Jugular Vein Conduit    [Abstract] Objective To evaluate the immunogenicity of different cross-linking methods tanned Bovine Jugular Vein Conduit(BJVC). MethodsThe BJVC tanned with glutaradehyde(GA) photo-oxidation(PH) polyepoxy compound(PC). The antigens which were obtained from BJVCs tanned with PC、GA、PH and fresh BJVC were mixed with  c